NEW USERS: To be trained as an independent user of the LSM 880 please contact Mr. Kennedy Nguyen, [email protected]. To receive authorization for independent use:
1) Undergo training
2) Provide information on the status of the student (graduate, postdoc, undergraduate), their year and major, the name of their advisor.
3) Submit a short paragraph describing the research being conducted with the LSM880.
In June, the PI team will contact all users to obtain a list of exemplary images, data and/or publications that have arisen from use of the LSM880. Priority for scheduling time may be subject to demonstration of research outcomes.
1) Undergo training
2) Provide information on the status of the student (graduate, postdoc, undergraduate), their year and major, the name of their advisor.
3) Submit a short paragraph describing the research being conducted with the LSM880.
In June, the PI team will contact all users to obtain a list of exemplary images, data and/or publications that have arisen from use of the LSM880. Priority for scheduling time may be subject to demonstration of research outcomes.
Publications, posters, and presentations that arise from data collected on the LSM 880 should acknowledge support from NSF using the following text:
The data in this work was collected, in part, with a confocal microscope acquired through the National Science Foundation MRI Award Number DMR-1625733.
Please also send a copy of the final published version of the paper to [email protected] for NSF reporting purposes.
The data in this work was collected, in part, with a confocal microscope acquired through the National Science Foundation MRI Award Number DMR-1625733.
Please also send a copy of the final published version of the paper to [email protected] for NSF reporting purposes.
Calendar
Six Excitation Laser Lines
405 nm , 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
Detectors
One Galium arsenide phosphide (GaAsP) photon counting photodetector (high sensitivity imaging, FCS, and FRET).
Airyscan+FAST detector
Two confocal PMTs
Transmitted light PMT
Airyscan+FAST detector
Two confocal PMTs
Transmitted light PMT
Contrast Mechanisms
Confocal Epifluorescence
Transmitted Differential Interference Contrast (DIC)
AiryScan
Transmitted Differential Interference Contrast (DIC)
AiryScan
Objectives on the LSM 880
1. Objective Fluar 2.5x/0.12 M27
2. Objective Plan-Apochromat 10x/0.45 M27
3. Objective W Plan-Apochromat 20x/1.0 DIC M27 75mm
4. Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27
5 .Objective Plan-Apochromat 63x/1.4 Oil DIC M27
These objectives are dedicated objectives on the LSM 880. They should not be removed from the LSM 880 room. If missing please contact a member of staff immediately.
2. Objective Plan-Apochromat 10x/0.45 M27
3. Objective W Plan-Apochromat 20x/1.0 DIC M27 75mm
4. Objective LD LCI Plan-Apochromat 40x/1.2 Imm Corr DIC M27
5 .Objective Plan-Apochromat 63x/1.4 Oil DIC M27
These objectives are dedicated objectives on the LSM 880. They should not be removed from the LSM 880 room. If missing please contact a member of staff immediately.
Other Objectives Available from the Subramaniam Lab
1. Objective "Plan-Neofluar" 10x/0.30 Pol
2. Objective Plan-Apochromat 20x/0.8 M27
These objectives belong to the Subramaniam Lab's LSM 700. While available for loan to trained users of the LSM 880, priority use of these objectives is for Subramaniam lab members and collaborators. The availability for use on the LSM 880 is not guaranteed without prior arrangement. Non-lab members should plan accordingly.
2. Objective Plan-Apochromat 20x/0.8 M27
These objectives belong to the Subramaniam Lab's LSM 700. While available for loan to trained users of the LSM 880, priority use of these objectives is for Subramaniam lab members and collaborators. The availability for use on the LSM 880 is not guaranteed without prior arrangement. Non-lab members should plan accordingly.